By G. Spiller
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Caffeine is the main commonly fed on drug on the planet, most typically from the drinks espresso, tea and soda. An predicted eighty percentage of the world's inhabitants consumes a caffeine-containing substance day-by-day. a standard 8-ounce (240-ml) cup of rapid espresso comprises approximately a hundred mg of caffeine - approximately two times up to a cup of tea or a 12-ounce (360-ml) can/bottle of soda.
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48,372,1993. ©1998 CRC Press LLC 25. Volatile constituents in alfalfa and red clover extracts, in Flavors and Fragrances: A World Perspective, Lawrence, B. M. , Conference. , 16-20 Nov. 1986, Elsevier Science Publishers, Amsterdam, The Netherlands, 1988. 26. , Analytical methods applied to coffee liqueur. III. Determination of furfural, caffeine and tannic substances, Tecnicas de Laboratorio, 12,204,1989. 27. , Gas chromatographic determination of theobromine in foods, J. Food Hygienic Soc. , 27(1),75,1986.
50 C. Gas Chromatography GC continues to have a great deal of use in clinical analysis. The literature contains a number of citations on the use of GC for the determination of theophylline in body fluids,51–53 but now it is possible to selectively determine not only theophylline in the presence of barbiturates, but also caffeine, theobromine, and other xanthine derivatives. The GC methods in the literature are used as a final determination step after extraction with organic solvents. It is, however, necessary to form a derivative of the methylxanthine, to use a flame-ionization (FID) or electron capture detector, and to use an internal standard.
Int. , 25,7,1970. 9. P. , Differential spectrophotometric determination of caffeine in soluble coffee and drug combinations, JAOAC, 51,573,1968. 10. C, 1980, p 234. 11. M. , Determination of caffeine using N-chloro-ptoluene-sulfonamide, Analyst, 106,729,19811 12. , Determination of caffeine by UV spectrometry in various types of coffee: green coffee and natural roasted coffee, Anales de Bromatologia, 42,241,1990. 13. , UV spectrophotometric determination of theobromine and caffeine in cocoa beans, Analytica Chimica Acta, 232,409,1990.
Caffeine by G. Spiller