By May S.
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2001). , 1989). , 2002). Therefore, much effort should be spent on the optimization of antigen retrieval, a work that may be more rewarding than changing dilutions of primary antibodies or detection systems. , 2003). In addition, the pH of the retrieval fluid rather than its chemical constituents seems to be important. , 1998). , 1997). The method of heating may also influence the performance. , 2001). Probably, the higher temperature reached because of excess pressure contributes to the better retrieval, and a loss of maximum temperature by a few degrees results in lower staining index of normal germinal centers for Ki-67.
Ye in Volume I of this series. Signal Transduction: Signal transduction describes the process by which a receptor interacts with a ligand at the surface of the cell and then transmits a signal to trigger a pathway within the cell. The basic principle of this interaction is that ligand binding on the extracellular side influences the activity of the receptor domain on the cytoplasmic side. The signal is transduced across the membrane. Signal transduction provides a means for amplification of the original signal.
To ensure optimal penetration of the fixative, large specimens should be incised before fixation, especially when encapsulated. , 2000). , 1997) or fixation in B5 or Bouin’s solution. , 1999). Fixation must be standardized in some settings intending to provide quantitative results, because standardized specimen size and fixation protocols are required in the FDA-approved HercepTest. Referral centers, however, cannot trust that all material they receive is fixed identically. Formalin fixation leads to cross-linking of proteins and therefore masks epitopes to which antibodies might bind.
A Cautionary Note on the Use of the Gronnesby and Borgan Goodness-of-fit Test for the Cox Proportional Hazards Mode by May S.